The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. 1. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. Fully tested with validations, warranty. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. Download PDF versions of the SpectraMax Microplate Reader User Guides. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Dual-Glo luciferase assay system (Promega Cat. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. : 2 Oct 12 Page 6 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Detection modalities include absorbance (UV-Vis Abs) and. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Add to Cart. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. 1. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). The plate. . Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Microplate reader settings. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. SpectraMax Microplate Readers. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. 315 can't find zero order. keeping with best practices, reader performance should. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. More Product Information. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax Spectrofluorometers. The system uses two scanning monochromators to determine the optimal excitation and emission settings. An enhancer is provided in the kit where applicable to further reduce background fluorescence and to increase the. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. Unlock your i3/i3x instrument after shipment. This system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. AlphaScreen. The SpectraMax® ABS Plus Microplate Reader can run . The Molecular Devices Gemini XPS Fluorescent Microplate Reader provides an ideal environment to determine the optimal excitation and emission settings for most fluorescent assays. Figure 1. Path Check Pathlength Measurement SpectraMax i3x. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 58% for the detection module and 2. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3 and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most publishedi3x. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. \ Figure 3. It measures the optical density (OD) of samples in both 96- and 384-well. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The drawing shows a standard microplate of 15 mm height covered with a lid. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12The optical system of SpectraMax Plus384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 1000 nm). 4 μL DNA standards. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. When the user applies a set temperature of 37°C, the top is heated to 37. When read on microplate readers, the SpectraMax Quant dsDNA Assay Kits enable optimal results and increased assay throughput. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. Here, we demonstrate performance of the SpectraMax® iD3 Multi-Mode Microplate Reader for assays measuring intrinsic tryptophan fluorescence. Published Dec 16, 2020 | Updated May 19, 2023. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. The SpectraMax® i3 is Molecular Devices latest multi-mode microplate reader which delivers several applications including; Ultra-Violet to visible absorbance detection, fluorescence intensity and multi-colour luminescence. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. The screw remains inside the instrument. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. Assays can be better optimized by scanning across. The analysis capabilities of the software provide quantitation in an easy-to-read, user customizable report format. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax® Mini reader is user-upgradeable - from two to three modes, providing researchers with a versatile instrument. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Download the SpectraMax Mini Reader Plug-in and associated guides. A. SpectraMax L Microplate Reader This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro. Add to Cart. . limit of detection (LLD) for ATP was estimated for each microplate reader using the . Fill a clean plate with water. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Other technologies such as imaging. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. Narrow 2nm bandwidths deliver increased measurement accuracy and linearity over the widest range. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications. SpectraMax® Microplate Reader Suite of Products Knowledge Base Published Dec 16, 2020 | Updated Dec 06, 2021 Products : SpectraMax i3x reader,. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. Summary of Contents for Molecular Devices SpectraMax M2. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. The drawing shows a standard microplate of 15 mm height covered with a lid. Due to elevated security of a Windows 7 64-bit operating system, the USB instrument drivers may not be installed automatically. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). It features a modular design that allows users to add or change detection cartridges according to their needs. Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. On the back, in the right hand top corner,. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Read one sample or up to 384 inThe SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. 3) At the bottom of the strip, click on "Show. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Molecular Devices: Molecular Devices. All stand-alone instrument functions can be controlled using the software. SpectraMax® Mini Multi-Mode Microplate Reader for Biotech Laboratories Built on the company’s nearly four decades of experience delivering high-performance microplate reader technology to the market, the SpectraMax Mini reader is robust enough to meet a lab’s core microplate reader needs, while its compact size is ideal for integration into a. SpectraMax Mini Plug-In for SoftMax Pro 7. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. The Spectramax 190 has an array of data collection modes, including kinetic, endpoint, and spectral scan modes. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. It was also read on the monochromator-based. . The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 58% for the detection module and 2. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. with SpectraMax Microplate Readers Introduction Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Or scan up to 384 wells at any time to determine the best assay conditions. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. Request a Quote!Introduction. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. 030 fmol Fluorescence Polarization (FP). SpectraMax Plus: SpectraMax Plus. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. Contact our support team with your questions regarding Molecular Devices products and applications. Molecular Devices. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. SpectraMax Glo Steady-Luc Reporter Assay Kit. e. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Lift up the lid over the chamber to insert or remove a cuvette. The SpectraMax L Luminescence Microplate Reader with the variable-volume injectors option (10 µL to 100 µL) can measure both flash and glow luminescence. The data suggests that there is little interference from dead cells when live cells are analyzed, however, there may be some interference from live cells when dead cells are analyzed. The guide also covers the software features, the specifications, and the accessories of the systems. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. The price range of a reader starts at 2. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. . . Detailed specifications of each model reader are reported in Section 7, “Specifications”. Supplier: Molecular Devices. The SpectraMax M2 and M2e are versatile and reliable. 0 out of 5 $ 11,999. Cell Cytotoxicity assay procedure. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. The plate reader can measure multiple readouts, including UV and visible absorbance. microplate reader. IMAP FP Settings in SoftMax Pro SpectraMax M5 Multi-Mode Microplate Reader. Additionally, user-installable options allow the SpectraMax i3 System to. Improve your lab’s efficiency, get more data, and decrease time to discovery with an automated microplate washer and reader solution: The SpectraMax® Mini Multi-Mode. , April 14, 2022 – Molecular Devices, LLC. The temperature in the plate chamber is ambient and isothermal. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The eight-channel system consists of 8 sample beams and 8 reference beams, delivering both superior precision and speed of reading across the microplate. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. The Spectramax 250s xenon flash lamp and grating monochromator facilitates direct UV measurement of DNA, RNA, and protein in microplates. Using the optimized wavelength settings, the SPECTRAmax GEMINI XS fluorescence microplate reader can distinguish between live and dead cells using this assay. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Offer Ends In: 2 Days 10: 40: 19. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 µL sample volume in a. Cell Analysis. Brochure. View the Molecular Devices website. Cuvette Microplate wells. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. The calculated LOQ values were 0. The plate reader can measure multiple readouts, including UV. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. These lab workhorses are used in drug discovery. flexibility of monochromator-based systems. 2. Figure 1. edu) to be added as a trained user in iLab. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. 2. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. This unlocks the transport slide. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Molecular Interactions. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. 0 out of 5 $ 5,999. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). microplate reader. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The plate was read on the SpectraMax Plus384 Absorbance Microplate Reader and SpectraMax i3x Multi-Mode Microplate Reader, using a preconfigured SpectraDrop protocol in SoftMax ® Pro Software. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. With optimized reagents, option to operate the. In . 0 out of 5 $ 23,995 $ 22,495. Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Temperature control and shaking come as standard. . Fluorescence analysis of the colonies from the dilution series was performed in a microplate reader (SpectraMax iD3). Examples: “Press carefully to engage,” “ Do not press down. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. . SAN JOSE, CA — Molecular Devices, LLC. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. Lower limits of detection for ATP determined using the ATPliteAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. SpectraMax M5 reader settings are shown. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Type:Spectrophotometer. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 0 CART STOREVersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. If it looks fine, then proceed. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. Italic and boldface type are used for emphasis. In addition, the SpectraMax® iD5 can be expanded to include bottom. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. 314 low lamp level warning. 2 only: SpectraMax Mini Update. When the user applies a set temperature of 37°C, the top is heated to 37. 314 low lamp level warning. 1. Examples: “Press carefully to engage,” “ Do not. Our specialization servicing. 72 MB. Products : General, Microplate Readers. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Data exemplifying linear ranges from the three different kits are presented in Figure 2. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. Step 2: Launch SoftMax Pro Software. Step 4: Incubate at room temperature for 1 hour, protected from light. 6% for the on-board optics (monochromators). Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. Nuclear Receptor. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. . The SpectraMax ® M5e Multi-Mode Microplate Reader is a dualmonochromator capable of fluorescence, time-resolved fluorescence (TRF), fluorescence polarization, absorbance, and luminescence assays. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. If you do not have an iLab account you must first make one: Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. limit of detection (LLD) for ATP was estimated for each microplate reader using the . 020 fmol 0. ELISA Plate Readers / Microplate Readers. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. Step 2: Launch SoftMax Pro Software. 315 can't find zero order. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. flexibility of monochromator-based systems. SpectraMax Microplate Readers. 0 out of 5 $ 11,999. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The SpectraMax microplate readers can be used to accurately quantify ROS levels using a luminescence-based assay. Step 1: Open the SoftMax Pro IMAP FP protocol. Add to Cart. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Plate chamber design of the SpectraMax category 1 microplate readers. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. com 4. Lysozyme contains two tryptophan residues that. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. SpectraMax Mini User Guide. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMinimum volume and maximum throughput with 96- or 384-well microplate compatibility. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Figure 6: Transcreener ADP2 FI assay principle. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. This unlocks the transport slide. 306 plate air cal. To resume collecting kinetic measurements within the same file, press the Append button. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Measuring platelet aggregation with a microplate reader. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. ; Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 380 nm/Exc. The powerful analysis tools in the software generate a standard curve and calculated data. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Microplate reader assays requiring infrared (IR) or red-shifted. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. Choose from a three- (M3), four- (M4), orIn this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Ease of use. In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. 1) Select the correct plate and the wells that you want to measure. The dual monochromator optics allow the widest range of. Microplate Readers Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. These robust readers have been placed in labs from. Lower limits of detection for ATP determined using the ATPlitePage 1 ® SPECTRAmax Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0085 Rev. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Molecular Devices. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership.